Reagent Preparation

Reagent Preparation Table

REAGENT	CHEMICAL WT / VOL	VOLUME AND MEDIA
4 % Boric acid	40 g	volume makeup 1000 ml with water
Rosalic acid	1 g	100 ml abslute alcohol
Alcohol 60 %	60 ml	vol makeup 100 ml with water
Sodium 100 ppm	0.1271 g Sodium Chloride	vol makeup 500 ml with water
Sodium 1000 ppm	1.2701 g Sodium Chloride	vol makeup 500 ml with water
Ammonia Buffer Solution	2.7g Ammonium Chloride (NH4Cl) + 17.8ml conc. Ammonia (NH3, 16M)	vol makeup 100 ml with water
Standard phosphate Solution 50 mg / ml	220 mg potassium di hydrozen phosphate	vol makeup 1000 ml with water
Nitric Acid 1.25 M	80 ml nitric acid	vol makeup 1000 ml with water
Phelopthaline indicator	1 g in 100 ml alcohol and heat	make up 200 ml with water
Rodamine dye	1 g in 100 ml abslute alcohol
Mixed indicator	0.2 g methyl red + 0.1 g mythelene blue	dissolve in 100 ml alcohol
Methyline blue	1 g	dissolve in 100 ml water
Starch indicator	0.5 g starch	50 ml boiling water
0.1 N Silver Nitrate	8.5 g AgNO3	dissolve in 500 ml water
0.01N Sodium Thio Sulphate	2.48 g	dissolve in 1000 ml water
Potassium permagnet Solution 2%	2 g	vol makeup 100 ml with water
0.1 N KOH	5.61 g	vol makeup 1000 ml with water
Antimony tri Chloride soln.	dissol 113.4g in 300-400 ml CHCl3 +5 g CaCl3 in hot condition and filter	vol makeup 500 ml with CHCl3
KOH 50 % w/v	50 g KOH	vol makeup 100 ml with water
Ammonium Vendate	2.5 g NH4VO4 in 500 ml hot water + 20 ml conc HNO3	vol makeup 1000 ml with water
TCA 10 %	100 g TCA	vol makeup 1000 ml with water
Chloroform acetic acid solution	20 ml acetic acid + 10ml chloroform
N/10 sulphuric acid	2.72 ml conc H2SO4	vol makeup 1000 ml with water
Nitric Acid 1.25 M	80 ml conc HNO3	vol makeup 1000 ml with water
N/50 Sulphuric Acid Solution	0.55 ml	vol makeup 1000 ml with water
Potassium Thiocynate 20% W/v	200 g KCN	vol makeup 1000 ml with water
Potassium per sulphate Solution 2 %	2 g	vol makeup 100 ml with water
Furfuraldehyde Solution 2%	2 ml furfuraldehyde freshly distilled	vol makeup 100 ml with alcohol
Suphuric Acid 1.25 %	12.5 ml conc H2SO4	vol makeup 1000 ml with water
Lacquer Solution	10 g CuSO4 + 20 ml conc HCl	vol makeup 100 ml with water
Potassium Chromate solution 5 %	5 g	vol makeup 100 ml with water
Sodium Carbonate 25 %	25 g	vol makeup 100 ml with water
Ferrous ammonium Sulphate 0.1 N	39 g in 500 ml water + 20 ml conc.	vol makeup 1000 ml with water
	H2SO4
Standard Potassium Chromate Soln	12.25 g	vol makeup 1000 ml with water
Digestion Mixture	25 g CuSO4 + 500 g K2SO4
Sulphuric Acid Reagent for COD	add 5.5 g AgSO4 per kg conc. H2SO4 stand for 1 -2 days
DMAB	1.60 g + 90 ml Ethyl Alcohol + 10 Hcl
Methyal Red incator	1 g	vol makeup 100 ml with alcohol
Standard Indophenol Solution	Dissolve 50 mg of sodium 2, 6-dichloro-benzenone indophenol, that has been stored in a dessicator over soda lime, in 50 ml of water to which 42 mg of sodium bi carbonate has been added. Shake vigorously and ,when the indophenol has completely dissolved, dilute it to 200 ml with water. Filter the solution through a fluted filter paper into an amber glass stoppered bottle. Keep the bottle stoppered, out of direct sunlight, and store in a refrigerator.