Reagent Preparation Table
| REAGENT | CHEMICAL WT / VOL | VOLUME AND MEDIA |
|---|---|---|
| 4 % Boric acid | 40 g | volume makeup 1000 ml with water |
| Rosalic acid | 1 g | 100 ml abslute alcohol |
| Alcohol 60 % | 60 ml | vol makeup 100 ml with water |
| Sodium 100 ppm | 0.1271 g Sodium Chloride | vol makeup 500 ml with water |
| Sodium 1000 ppm | 1.2701 g Sodium Chloride | vol makeup 500 ml with water |
| Ammonia Buffer Solution | 2.7g Ammonium Chloride (NH4Cl) + 17.8ml conc. Ammonia (NH3, 16M) | vol makeup 100 ml with water |
| Standard phosphate Solution 50 mg / ml | 220 mg potassium di hydrozen phosphate | vol makeup 1000 ml with water |
| Nitric Acid 1.25 M | 80 ml nitric acid | vol makeup 1000 ml with water |
| Phelopthaline indicator | 1 g in 100 ml alcohol and heat | make up 200 ml with water |
| Rodamine dye | 1 g in 100 ml abslute alcohol | |
| Mixed indicator | 0.2 g methyl red + 0.1 g mythelene blue | dissolve in 100 ml alcohol |
| Methyline blue | 1 g | dissolve in 100 ml water |
| Starch indicator | 0.5 g starch | 50 ml boiling water |
| 0.1 N Silver Nitrate | 8.5 g AgNO3 | dissolve in 500 ml water |
| 0.01N Sodium Thio Sulphate | 2.48 g | dissolve in 1000 ml water |
| Potassium permagnet Solution 2% | 2 g | vol makeup 100 ml with water |
| 0.1 N KOH | 5.61 g | vol makeup 1000 ml with water |
| Antimony tri Chloride soln. | dissol 113.4g in 300-400 ml CHCl3 +5 g CaCl3 in hot condition and filter | vol makeup 500 ml with CHCl3 |
| KOH 50 % w/v | 50 g KOH | vol makeup 100 ml with water |
| Ammonium Vendate | 2.5 g NH4VO4 in 500 ml hot water + 20 ml conc HNO3 | vol makeup 1000 ml with water |
| TCA 10 % | 100 g TCA | vol makeup 1000 ml with water |
| Chloroform acetic acid solution | 20 ml acetic acid + 10ml chloroform | |
| N/10 sulphuric acid | 2.72 ml conc H2SO4 | vol makeup 1000 ml with water |
| Nitric Acid 1.25 M | 80 ml conc HNO3 | vol makeup 1000 ml with water |
| N/50 Sulphuric Acid Solution | 0.55 ml | vol makeup 1000 ml with water |
| Potassium Thiocynate 20% W/v | 200 g KCN | vol makeup 1000 ml with water |
| Potassium per sulphate Solution 2 % | 2 g | vol makeup 100 ml with water |
| Furfuraldehyde Solution 2% | 2 ml furfuraldehyde freshly distilled | vol makeup 100 ml with alcohol |
| Suphuric Acid 1.25 % | 12.5 ml conc H2SO4 | vol makeup 1000 ml with water |
| Lacquer Solution | 10 g CuSO4 + 20 ml conc HCl | vol makeup 100 ml with water |
| Potassium Chromate solution 5 % | 5 g | vol makeup 100 ml with water |
| Sodium Carbonate 25 % | 25 g | vol makeup 100 ml with water |
| Ferrous ammonium Sulphate 0.1 N | 39 g in 500 ml water + 20 ml conc. | vol makeup 1000 ml with water |
| H2SO4 | ||
| Standard Potassium Chromate Soln | 12.25 g | vol makeup 1000 ml with water |
| Digestion Mixture | 25 g CuSO4 + 500 g K2SO4 | |
| Sulphuric Acid Reagent for COD | add 5.5 g AgSO4 per kg conc. H2SO4 stand for 1 -2 days | |
| DMAB | 1.60 g + 90 ml Ethyl Alcohol + 10 Hcl | |
| Methyal Red incator | 1 g | vol makeup 100 ml with alcohol |
| Standard Indophenol Solution | Dissolve 50 mg of sodium 2, 6-dichloro-benzenone indophenol, that has been stored in a dessicator over soda lime, in 50 ml of water to which 42 mg of sodium bi carbonate has been added. Shake vigorously and ,when the indophenol has completely dissolved, dilute it to 200 ml with water. Filter the solution through a fluted filter paper into an amber glass stoppered bottle. Keep the bottle stoppered, out of direct sunlight, and store in a refrigerator. |